Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli

Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli.

In recent years, the prospect of using Mycobacterium bovis BCG as a live vaccine carrier for foreign antigens has prompted a rapid evolution of the molecular biology of Mycobacteria. This progress was considerably facilitated by the development of the electroporation technique allowing high levels of transformation rates of this organism (1). Nowadays, Escherichia coliMycobacteria shuttle plasm...

Study of antimicrobial susceptibility and plasmid analysis of Escherichia coli in Iran, Urmia

F'Cm Plasmid of Escherichia coli

A strain of Pasteurella pestis, harboring the F'Cm plasmid from Escherichia coli, was able to donate its chromosome to auxotrophic recipient strains of P. pestis. The frequency of gene transfer in P. pestis was approximately 10-6 per donor cell, 100 times less efficient than gene transfer in Pasteurella pseudotuberculosis, but efficient enough to determine entry times for the markers histidine,...

Citrate utilization by Escherichia coli: plasmid- and chromosome-encoded systems.

Citrate utilization plasmids have previously been identified in atypical Escherichia coli isolates. A different citrate-utilizing (Cit+) variant of E. coli K-12 arose as a consequence of two chromosomal mutations (B. G. Hall, J. Bacteriol. 151:269-273, 1982). The processes controlling the transport of citrate in both a Cit+ chromosomal mutant and a Cit+ plasmid system were studied. Both systems...

Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid

We report here the complete nucleotide sequence of pEntH10407 (65 147 bp), an enterotoxigenic Escherichia coli enterotoxin plasmid (Ent plasmid), which is self-transmissible at low frequency. Within the plasmid, we identified 100 open reading frames (ORFs) which could encode polypeptides. These ORFs included regions encoding heat-labile (LT) and heat-stable (STIa) enterotoxins, regions encoding...